Cellular and molecular characterization of Phytophthora parasitica appressorium-mediated penetration.
Identifieur interne : 001970 ( Main/Exploration ); précédent : 001969; suivant : 001971Cellular and molecular characterization of Phytophthora parasitica appressorium-mediated penetration.
Auteurs : N. Kebdani [France] ; L. Pieuchot ; E. Deleury ; F. Panabières ; J-Y Le Berre ; M. GourguesSource :
- The New phytologist [ 1469-8137 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN des algues (MeSH), Banque de gènes (MeSH), Cellules végétales (MeSH), Maladies des plantes (MeSH), Phytophthora (génétique), Phytophthora (pathogénicité), Phytophthora (physiologie), Plantes (parasitologie), Protéines d'algue (génétique), Protéines d'algue (métabolisme), Racines de plante (cytologie), Racines de plante (parasitologie), Racines de plante (physiologie).
- MESH :
- cytologie : Racines de plante.
- génétique : Phytophthora, Protéines d'algue.
- métabolisme : Protéines d'algue.
- parasitologie : Plantes, Racines de plante.
- pathogénicité : Phytophthora.
- physiologie : Phytophthora, Racines de plante.
- ADN des algues, Banque de gènes, Cellules végétales, Maladies des plantes.
English descriptors
- KwdEn :
- Algal Proteins (genetics), Algal Proteins (metabolism), DNA, Algal (MeSH), Gene Library (MeSH), Phytophthora (genetics), Phytophthora (pathogenicity), Phytophthora (physiology), Plant Cells (MeSH), Plant Diseases (MeSH), Plant Roots (cytology), Plant Roots (parasitology), Plant Roots (physiology), Plants (parasitology).
- MESH :
- chemical , genetics : Algal Proteins.
- chemical , metabolism : Algal Proteins.
- chemical : DNA, Algal.
- cytology : Plant Roots.
- genetics : Phytophthora.
- parasitology : Plant Roots, Plants.
- pathogenicity : Phytophthora.
- physiology : Phytophthora, Plant Roots.
- Gene Library, Plant Cells, Plant Diseases.
Abstract
Data on plant pathogenic oomycetes are scarce and little is known about the early events leading to the onset of infection. The aim of this work was to analyze the penetration process of the soil-borne plant pathogen Phytophthora parasitica, which has a wide host range. Here, we performed a cytological analysis of the colonization of the first plant cell and developed an inoculation assay for characterizing the entire penetration process through cellular and molecular analyses. We showed that P. parasitica infects roots by producing a specialized structure, the appressorium. We produced the first cDNA library for the penetrating stage of a Phytophthora species and showed it to be highly enriched in pathogenicity-related sequences. These included coding sequences for many cell-degrading enzymes, effectors such as RXLR-containing proteins and proteins involved in protection against plant defense responses. Characterization of the appressorium cDNA library and identification of genes overrepresented early in P. parasitica infection provided us with an unprecedented opportunity to decipher the molecular mechanisms involved in penetration of the plant cells during the initiation of infection by a soil-borne oomycete.
DOI: 10.1111/j.1469-8137.2009.03048.x
PubMed: 19807870
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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The aim of this work was to analyze the penetration process of the soil-borne plant pathogen Phytophthora parasitica, which has a wide host range. Here, we performed a cytological analysis of the colonization of the first plant cell and developed an inoculation assay for characterizing the entire penetration process through cellular and molecular analyses. We showed that P. parasitica infects roots by producing a specialized structure, the appressorium. We produced the first cDNA library for the penetrating stage of a Phytophthora species and showed it to be highly enriched in pathogenicity-related sequences. These included coding sequences for many cell-degrading enzymes, effectors such as RXLR-containing proteins and proteins involved in protection against plant defense responses. Characterization of the appressorium cDNA library and identification of genes overrepresented early in P. parasitica infection provided us with an unprecedented opportunity to decipher the molecular mechanisms involved in penetration of the plant cells during the initiation of infection by a soil-borne oomycete.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19807870</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>185</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Jan</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Cellular and molecular characterization of Phytophthora parasitica appressorium-mediated penetration.</ArticleTitle><Pagination><MedlinePgn>248-57</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2009.03048.x</ELocationID><Abstract><AbstractText>Data on plant pathogenic oomycetes are scarce and little is known about the early events leading to the onset of infection. The aim of this work was to analyze the penetration process of the soil-borne plant pathogen Phytophthora parasitica, which has a wide host range. Here, we performed a cytological analysis of the colonization of the first plant cell and developed an inoculation assay for characterizing the entire penetration process through cellular and molecular analyses. We showed that P. parasitica infects roots by producing a specialized structure, the appressorium. We produced the first cDNA library for the penetrating stage of a Phytophthora species and showed it to be highly enriched in pathogenicity-related sequences. These included coding sequences for many cell-degrading enzymes, effectors such as RXLR-containing proteins and proteins involved in protection against plant defense responses. Characterization of the appressorium cDNA library and identification of genes overrepresented early in P. parasitica infection provided us with an unprecedented opportunity to decipher the molecular mechanisms involved in penetration of the plant cells during the initiation of infection by a soil-borne oomycete.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kebdani</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>UMR Interactions Biotiques et Santé Végétale, INRA1301-CNRS6243-UNSA1301, 400 route des chappes, F-06903 Sophia Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pieuchot</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Deleury</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Panabières</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Le Berre</LastName><ForeName>J-Y</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Gourgues</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>10</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020418">Algal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D048028">DNA, Algal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020418" MajorTopicYN="N">Algal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D048028" MajorTopicYN="Y">DNA, Algal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="Y">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19807870</ArticleId><ArticleId IdType="pii">NPH3048</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2009.03048.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Sophia Antipolis</li></settlement></list><tree><noCountry><name sortKey="Deleury, E" sort="Deleury, E" uniqKey="Deleury E" first="E" last="Deleury">E. Deleury</name><name sortKey="Gourgues, M" sort="Gourgues, M" uniqKey="Gourgues M" first="M" last="Gourgues">M. Gourgues</name><name sortKey="Le Berre, J Y" sort="Le Berre, J Y" uniqKey="Le Berre J" first="J-Y" last="Le Berre">J-Y Le Berre</name><name sortKey="Panabieres, F" sort="Panabieres, F" uniqKey="Panabieres F" first="F" last="Panabières">F. Panabières</name><name sortKey="Pieuchot, L" sort="Pieuchot, L" uniqKey="Pieuchot L" first="L" last="Pieuchot">L. Pieuchot</name></noCountry><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Kebdani, N" sort="Kebdani, N" uniqKey="Kebdani N" first="N" last="Kebdani">N. Kebdani</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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